About The Payne Lab
The Payne Lab's studies focus on the genetics and regulation of iron acquisition systems and other virulence factors of the intestinal pathogens Shigella flexneri and Vibrio cholerae. The Lab's work combines biochemistry and genetics to determine how the essential metals iron and manganese are transported and utilized in V. cholerae and S. flexneri. Iron transport systems are of particular interest to Payne Lab researchers because iron acquisition in the human host is critical to microbial virulence. Specifically they are studying the bacterial Feo system for ferrous iron transport. Additionally, Lab scientists use in vitro models combined, such as enteroids, with genetic analyses to determine the role of host lipids and carbon metabolism in the pathogenesis of S. flexneri. Regarding S. flexneri, they also examine interspecies interactions between S. flexneri and normal members of human gut microbiota to determine how they influence in S. flexneri growth and virulence. Finally, The Payne Lab is also exploring the environmental regulation of critical virulence factors by the RNA binding protein CsrA in V. cholerae.
2023 Research
- Kago G, Turnbough CL Jr, Salazar JC, Payne SM. (p)ppGpp is required for virulence of Shigella flexneri. Infect Immun. 2023 Dec 15:e0033423. Epub ahead of print. PMID: 38099658.
- Gómez-Garzón C, Payne SM. Divide and conquer: genetics, mechanism, and evolution of the ferrous iron transporter Feo in Helicobacter pylori. Front Microbiol. 2023 Jul 4;14:1219359. PMID: 37469426; PMCID: PMC10353542.
- Kostiuk B, Becker ME, Churaman CN, Black JJ, Payne SM, Pukatzki S, Koestler BJ. Vibrio cholerae Alkalizes Its Environment via Citrate Metabolism to Inhibit Enteric Growth In Vitro. Microbiol Spectr. 2023 Mar 14;11(2):e0491722. Epub ahead of print. PMID: 36916917; PMCID: PMC10100763.
2022 Research
- Xerri NL, Payne SM. Bacteroides thetaiotaomicron Outer Membrane Vesicles Modulate Virulence of Shigella flexneri. mBio. 2022 Oct 26;13(5):e0236022. Epub 2022 Sep 14. PMID: 36102517; PMCID: PMC9600379.
- C Gómez-Garzón, JE Barrick and SM Payne. Disentangling the Evolutionary History of Feo, the Major Ferrous Iron Transport System in Bacteria.mBio. 11;13(1):e0351221. January 11, 2022.
2021 Research
- AR Mey, C Gómez-Garzón and SM Payne. Iron Transport and Metabolism in Escherichia, Shigella, and Salmonella. EcoSal Plus. 9(2):eESP-0034. December 15, 2021.
- M Shin M, C Gómez-Garzón and SM Payne. Vanadate inhibits Feo-mediated iron transport in Vibrio cholerae. Metallomics.13 (11) :mfab059. October 21, 2021.
- M Shin, D Mun, J Choi, S Kim, SM Payne and Y Kim. Identification of a New Antimicrobial Agent against Bovine Mastitis-Causing Staphylococcus aureus. J. Agric. Food Chem. 69 (34) :9968–9978. August 18, 2021.
- HA Butz, A Mey, AL Ciosek, AA Crofts, BW Davies and SM Payne. Regulatory Effects of CsrA in Vibrio cholerae . mBio. 12 (1) :e03380-20. February 2, 2021.
2020 Research
- M Shin M, Y Jin, J Park, D Mun, SR Kim, SM Payne, HK Kim and Y Kim. Characterization of an Antibacterial Agent Targeting Ferrous Iron Transport Protein FeoB against Staphylococcus aureus and Gram-Positive Bacteria. mBio. 16 (1) :136-149. December 30, 2021.
- C Gómez-Garzón and SM Payne. Vibrio cholerae FeoB hydrolyzes ATP and GTP in vitro in the absence of stimulatory factors. Metallomics. November 11, 2020.
- J Park, S Minhye, Y Jin, IJ Kim, SM Payne and KH Kim. Biochemical characterization of bacterial FeoBs: A perspective on nucleotide specificity. Arch. Biochem. Biophys. May 30, 2020 :108350.