About The Ippolito Lab
Current research of the Ippolito Lab utilizes molecular and proteomic techniques for the comprehensive profiling of antibody repertoires in human adaptive immune responses. The method is a synergistic combination of (i) IgG immunoglobulin protein mass spectrometry and (ii) a high-throughput DNA sequencing method that preserves the natural pairing of B-cell heavy and light chain variable regions (VH:VL regions). This innovation allows for the direct comparison of protein-level plasma IgG immunoglobulin repertoires to DNA-level repertoires in circulating peripheral blood B cells—a unique description that is yielding fresh insights into the temporal dynamics and sequence composition of antibody-mediated immunity. These techniques have been applied to the analysis of vaccine responses, autoimmunity, cancer, and infectious diseases like COVID-19. Two key technologies, BCR-Seq and Ig-Seq, are used in concert to gather immune response data that is then subjected to bioinformatic analysis.
2023 Research
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Melo-Braga MN, Carvalho MB, Ferreira MCE, Lavinder J, Abbasi A, Palmisano G, Thaysen-Andersen M, Sajadi MM, Ippolito GC, Felicori LF. Unveiling the multifaceted landscape of N-glycosylation in antibody variable domains: Insights and implications. Int J Biol Macromol. 2023 Nov 28;257(Pt 1):128362. Epub ahead of print. PMID: 38029898.
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Ippolito GC. Illuminating the dark side of neuraminidase in influenza immunity. Immunity. 2023 Nov 14;56(11):2463-2465. PMID: 37967527.
2022 Research
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Kuraoka M, Curtis NC, Watanabe A, Tanno H, Shin S, Ye K, Macdonald E, Lavidor O, Kong S, Von Holle T, Windsor I, Ippolito GC, Georgiou G, Walter EB, Kelsoe G, Harrison SC, Moody MA, Bajic G, Lee J. Infant Antibody Repertoires during the First Two Years of Influenza Vaccination. mBio. 2022 Oct 31:e0254622. Epub ahead of print. PMID: 36314798.
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Navas C, Manso T, Martins F, Minto L, Moreira R, Minozzo J, Antunes B, Vale A, McDaniel JR, Ippolito GC, Felicori LF. The major role of junctional diversity in the horse antibody repertoire. Mol Immunol. 2022 Nov;151:231-241. Epub 2022 Sep 27. PMID: 36179605.
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SJ Gonzales, KN Clarke, G Batugedara, R Garza, AE Braddom, RA Reyes, I Ssewanyana, KC Garrison, GC Ippolito, B Greenhouse, S Bol, EM Bunnik. A Molecular Analysis of Memory B Cell and Antibody Responses Against Plasmodium falciparum Merozoite Surface Protein 1 in Children and Adults From Uganda. Front Immunol. June 2, 2022.
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MV Gadush, GA Sautto, H Chandrasekaran, A Bensussan, TM Ross, GC Ippolito, MD Person. Template-Assisted De Novo Sequencing of SARS-CoV-2 and Influenza Monoclonal Antibodies by Mass Spectrometry. J Proteome Res. June 2, 2022.
2021 Research
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JJ Lavinder and GC Ippolito. Boosted immunity to the common cold might protect children from COVID-19. Nat Immunol. December 22, 2021.
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JM Schaub, CW Chou, HC Kuo, K Javanmardi, CL Hsieh, J Goldsmith, AM DiVenere, KC Le, D Wrapp, PO Byrne, CK Hjorth, NV Johnson, J Ludes-Meyers, AW Nguyen, N Wang, JJ Lavinder, GC Ippolito, JA Maynard, JS McLellan, IJ Finkelstein. Expression and characterization of SARS-CoV-2 spike proteins. Nat Protoc. 16(11):5339-5356. October 5, 2021.
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J Jung, ST Mundle, IV Ustyugova, AP Horton, DR Boutz, S Pougatcheva, P Prabakaran, JR McDaniel, GR King, D Park, MD Person, C Ye, B Tan, Y Tanno, JE Kim, NC Curtis, J DiNapoli, S Delagrave, TM Ross, GC Ippolito, H Kleanthous, J Lee, G Georgiou. Influenza vaccination in the elderly boosts antibodies against conserved viral proteins and egg-produced glycans. J Clin Invest. 131(13):e148763. July 1, 2021.
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J Li, SA Bazzi, F Schmitz, H Tanno, JR McDaniel, CH Lee, C Joshi, JE Kim, N Monson, BM Greenberg, K Hedfalk, E * Melamed, GC* Ippolito. Molecular Level Characterization of Circulating Aquaporin-4 Antibodies in Neuromyelitis Optica Spectrum Disorder. Neurol Neuroimmunol Neuroinflamm. 8(5):e1034. *co-corresponding authors. June 24, 2021.
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WN Voss, YJ Hou, NV Johnson, G Delidakis, JE Kim, K Javanmardi, AP Horton, F Bartzoka, CJ Paresi, Y Tanno, CW Chou, SA Abbasi, W Pickens, K George, DR Boutz, DM Towers, JR McDaniel, D Billick, J Goike, L Rowe, D Batra, J Pohl, J Lee, S Gangappa, S Sambhara, M Gadush, N Wang, MD Person, BL Iverson, JD Gollihar, J Dye, A Herbert, IJ Finkelstein, RS Baric, JS McLellan, G Georgiou, JJ* Lavinder, GC* Ippolito. Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes. Science. 372(6546):1108-1112. *co-corresponding authors. May 4, 2021.
2020 Research
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E Salazar, KK Perez, M Ashraf, J Chen, B Castillo, PA Christensen, T Eubank, DW Bernard, TN Eagar, SW Long, S Subedi, RJ Olsen, C Leveque, MR Schwartz, M Dey, C Chavez-East, J Rogers, A Shehabeldin, D Joseph, G Williams, K Thomas, F Masud, C Talley, KG Dlouhy, BV Lopez, C Hampton, J Lavinder, JD Gollihar, AC Maranhao, GC Ippolito, MO Saavedra, CC Cantu, P Yerramilli, L Pruitt, JM Musser. Treatment of COVID-19 Patients with Convalescent Plasma, American Journal of Pathology S0002-9440(20)30257-1. August 1, 2020.
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CL Hsieh, JA Goldsmith, JM Schaub, AM DiVenere, HC Kuo, K Javanmardi, KC Le, D Wrapp, AG Lee, Y Liu, CW Chou, PO Byrne, CK Hjorth, NV Johnson, J Ludes-Meyers, AW Nguyen, J Park, N Wang, D Amengor, JJ Lavinder, GC Ippolito, JA Maynard, IJ Finkelstein, JS McLellan. Structure-based design of prefusion-stabilized SARS-CoV-2 spikes, Science 369(6510):1501-1505. July 23, 2020.
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C Soni, OA Perez, WN Voss, L Serpas, J Mehl, J Goike, G Georgiou, GC Ippolito, V Sisirak, B Reizis. Plasmacytoid dendritic cells promote extrafollicular anti-DNA responses in lupus through type I interferon, Immunity 52(6):1022-1038. June 16, 2020.
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H Tanno, JR McDaniel, CA Stevens, WN Voss, J Li, R Durrett, J Lee, J Gollihar, Y Tanno, G Delidakis, A Pothukuchy, JW Ellefson, JJ Goronzy, JA Maynard, AD Ellington, GC Ippolito, G Georgiou. A facile technology for the high-throughput sequencing of the paired VH:VL and TCRβ:TCRα repertoires, Science Advances eaay9093. April 22, 2020.
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S Sterrett, BJ Peng, RL Burton, DC LaFon, AO Westfall, S Singh, M Pride, AS Anderson, GC Ippolito, HW Schroeder Jr, MH Nahm, AK Prasad, P Goepfert, A Bansal. Peripheral CD4 T follicular cells induced by a conjugated pneumococcal vaccine correlate with enhanced opsonophagocytic antibody responses in younger individuals, Vaccine 38(7):1778-1786. February 11, 2020.